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Standard Semaglutide Sodium Research Protocols

There is a standard protocol when engaging in research using semaglutide sodium.

Table of Contents

  • Reconstitution
    • Reconstitution of 5mg Vial
    • Reconstitution of 2mg Vial
  • Standard dose protocol for research

Reconstitution

Here are the reconstitution procedures for semaglutide sodium:

Reconstitution of 5mg Vial

In order to obtain standard dosing, the reconstitution ratio is two milliliters (2ml) of bacteriostatic water in 5mg of semaglutide sodium.

Supplies needed:

  • One (1) 5mg vial of semaglutide sodium
  • One (1) bottle of bacteriostatic water
  • One (1) syringe of at least 2ml in volume or two (2) syringes of 1ml in volume.
  • Several alcohol prep pads for disinfection use

Procedure:

  1. Place all components on a sterile surface.
  2. Remove cap from the 5mg semaglutide sodium vial.
  3. Remove cap from the bottle of bacteriostatic water.
  4. Using an alcohol prep pad, sterilize the now exposed top of the 5mg semaglutide sodium vial.
  5. Using an alcohol prep pad, sterilize the now exposed top of the bacteriostatic water bottle.
  6. Using a fresh syringe, extract 2ml of bacteriostatic water from the bottle.
  7. Add the bacteriostatic water into the 5mg semaglutide sodium vial.
    • If using a 1ml syringe, repeat steps 5, 6, and 7 with 1ml of water each time until the 5mg semaglutide sodium vial contains 2ml of bacteriostatic water.
    • In order to prevent cross contamination, do not use the same syringe twice.
  8. Once 2ml has been added to the 5mg semaglutide sodium vial, remove the syringe and dispose of it safely.
  9. Gently roll the vial to dissolve the semaglutide sodium into the bacteriostatic water. DO NOT SHAKE. Gently roll until dissolved entirely.
  10. Store the reconstituted solution in a refrigerated environment (DO NOT FREEZE.)

Once reconstituted, the solution has a shelf life of no longer than 8 weeks (56 days.)

After 8 weeks, dispose of the vial safely and appropriately.

Reconstitution of 2mg Vial

In order to obtain standard dosing, the reconstitution ratio is 0.8 milliliters (0.8ml) of bacteriostatic water in 2mg of semaglutide sodium. This is equal to 80 units on a standard syringe.

Supplies needed:

  • One (1) 2mg vial of semaglutide sodium
  • One (1) bottle of bacteriostatic water
  • One (1) syringe of at least 1ml in volume
  • Several alcohol prep pads for disinfection use

Procedure:

  1. Place all components on a sterile surface.
  2. Remove cap from the 2mg semaglutide sodium vial.
  3. Remove cap from the bottle of bacteriostatic water.
  4. Using an alcohol prep pad, sterilize the now exposed top of the 2mg semaglutide sodium vial.
  5. Using an alcohol prep pad, sterilize the now exposed top of the bacteriostatic water bottle.
  6. Using a fresh syringe, extract 0.8ml (80 units) of bacteriostatic water from the bottle.
  7. Add the bacteriostatic water into the 2mg semaglutide sodium vial.
  8. Once 0.8ml (80 units) has been added to the 2mg semaglutide sodium vial, remove the syringe and dispose of it safely.
  9. Gently roll the vial to dissolve the semaglutide sodium into the bacteriostatic water. DO NOT SHAKE. Gently roll until dissolved entirely.
  10. Store the reconstituted solution in a refrigerated environment (DO NOT FREEZE.)

Once reconstituted, the solution has a shelf life of no longer than 8 weeks (56 days.)

After 8 weeks, dispose of the vial safely and appropriately.

Standard Dose Protocol for Research

The standard dose schedule of reconstituted semaglutide sodium for academic research:

  • Week 1, 2, 3, 4 – 0.25mg of semaglutide sodium (10 units on a standard syringe)
  • Week 5, 6, 7, 8 – 0.5mg of semaglutide sodium (20 units on a standard syringe)
  • Week 9, 10, 11, 12 – 1.0mg of semaglutide sodium (40 units on a standard syringe)
  • Week 13, 14, 15, 16 – 1.7mg of semaglutide sodium (68 units on a standard syringe)
  • Week 17 and onward – 2.4mg of semaglutide sodium (96 units on a standard syringe)

For any help regarding dosing on a standard syringe, we highly recommend using peptidecalc.com

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